EMPIAR-11630 - cGMP-bound SpSLC9C1 in lipid nanodiscs [multiple data sets in TIFF format]

EMPIAR-11630

cGMP-bound SpSLC9C1 in lipid nanodiscs [multiple data sets in TIFF format]

Publication:

Structures of a sperm-specific solute carrier gated by voltage and cAMP

Kalienkova V, Peter MF, Rheinberger J, Paulino C

Nature 623 (2023) 202-209

PMID: 37880361

DOI: 10.1038/s41586-023-06629-w

Related EMDB entry:

EMD-17622 EMD-17621

Deposited:

2023-07-10

Released:

2023-11-14

Last modified:

2023-11-14

Imageset size:

1.62 TB

Imageset DOI:

10.6019/EMPIAR-11630

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movie frames of cGMP-bound SpSLC9C1 in nanodiscs, dataset 1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1438

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.022, 1.022)

Details:

Unaligned movie frames (60 frames per movie), collected at 200 kV in counting mode. Total electron dose is 50.189 electrons/Å^2. Data were recorded with SerialEM using 3 x 3 multi-shot pattern. Movies are not gain-corrected, gain reference is provided.

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2. Unaligned movie frames of cGMP-bound SpSLC9C1 in nanodiscs, dataset 2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3133

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.022, 1.022)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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3. Unaligned movie frames of cGMP-bound SpSLC9C1 in nanodiscs, dataset 3

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.022, 1.022)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Unaligned movie frames of cGMP-bound SpSLC9C1 in nanodiscs, dataset 4

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2239

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.022, 1.022)

Details:

Unaligned movie frames (60 frames per movie), collected at 200 kV in counting mode. Total electron dose is 53.599 electrons/Å^2. Data were recorded with SerialEM using 3 x 3 multi-shot pattern. Movies are not gain-corrected, gain reference is provided.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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