EMPIAR-11602 - Movies of Glycine/Glutamate bound GluN1a-GluN2D NMDAR [17718 multi-frame micrographs composed of 30 frames each in TIFF format]

EMPIAR-11602

Movies of Glycine/Glutamate bound GluN1a-GluN2D NMDAR [17718 multi-frame micrographs composed of 30 frames each in TIFF format]

Publication:

Structural insights into assembly and function of GluN1-2C, GluN1-2A-2C, and GluN1-2D NMDARs

Chou TH, Kang H, Simorowski N, Traynelis SF, Furukawa H

Molecular cell 82 (2022) 4548-4563.e4

PMID: 36309015

DOI: 10.1016/j.molcel.2022.10.008

Related EMDB entry:

EMD-27957

Deposited:

2023-06-19

Released:

2023-07-11

Last modified:

2023-07-11

Imageset size:

3.54 TB

Imageset DOI:

10.6019/EMPIAR-11602

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Movies of Glycine/Glutamate bound GluN1a-GluN2D NMDAR

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

17718

Image size:

(4092, 5760)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.856, 0.856)

Details:

The movies were collected at 300kV Titan with 30 frames. The total exposure dose was set to 74 e/A^2, spherical aberration at 2.7 mm, and the magnification at a pixel size of 0.856 Ang. Gain reference is also in the data folder and needs to be supplied when importing the movies. When using Cryosparc, X,Y axis needs to be flipped and rotated 3 times to fit the actual movies.

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