EMPIAR-11580
Molecular model of a bacterial flagellar motor in situ reveals a “parts-list” of protein adaptations to increase torque [multiple data sets in MRC format]
Publication:

Molecular model of a bacterial flagellar motor in situ reveals a “parts-list” of protein adaptations to increase torque

Beeby M, Drobnič T

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Related EMDB entry:
Deposited:
2023-02-03
Released:
2024-08-26
Last modified:
2024-08-26
Imageset size:
10.09 TB
Imageset DOI:
Experimental metadata:
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Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

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Contains:
  • micrographs - multiframe
1. Dataset 2 (“K2”)
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
43006
Image size:
(3838, 3710)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(2.2, 2.2)
Details:
Campylobacter_K2_20201001_151628_EPU

64,000 np EFTEM
2.2 A/pix
20eV slit
Spot 7
1.50 um beam size
17 s exp
32 frames
Dose rate: 14 e/p/s, 2.89 e/A2/s
50 e/A2 total dose
1.53 e/A2/frame
-1.5 um to -3.0 um defocus
Autofocus at 105,000 nP (1.05 um beam size)
0.6/1 quantifoil grids
To detect holes in EPU, use 9mm defocus in GridSq mode and "allow dark holes"
One shot per hole
Files:
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2. Dataset 1 (“F3”)
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
8775
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.748, 1.748)
Details:
campylobacter_mini_20200611_131510_EPU

47,000 nP
1.75 A/pix
50um C2
100um obj
spot 4
beam size: 1.3um
defocus range: -1.5 to -3.0
1.02 s exposure
10 movie frames
dose rate: 153 e/p/s
total dose: 50 e/A2
dose per movie frame: 5 e/A2/frame
Files:
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