EMPIAR-11570
HIV-1 capsid-like particles assembled from purified capsid protein and assembly cofactor IP6 [1487 multi-frame micrographs composed of 50 frames each in TIFF format]
Publication:

Structural insights into HIV-1 polyanion-dependent capsid lattice formation revealed by single particle cryo-EM

Highland CM, Tan A, Ricaña CL, Briggs JAG, Dick RA

Proceedings of the National Academy of Sciences of the United States of America 120 (2023)

PMID: 37094124

Related EMDB entry:
Deposited:
2023-04-05
Released:
2023-06-16
Last modified:
2023-06-16
Imageset size:
818.77 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of HIV-1 capsid-like particles
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1487
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.655, 0.655)
Details:
'Raw,' unaligned multi-frame movies collected using a Talos Arctica microscope equipped with a Gatan K3 detector/BioQuantum energy filter operating in super-resolution mode.

Voltage: 200 kV
Physical pixel size: 1.31Å/pixel
Total dose: 50 e-/Å^2

Gain reference (gain_ref.mrc) is included.
Picked particle coordinates are provided in run_data.star.
Files:
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Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)