EMPIAR-11510 - Cryo-EM structure of human pseudouridine synthase 3 [8211 micrographs in TIFF format]

EMPIAR-11510

Cryo-EM structure of human pseudouridine synthase 3 [8211 micrographs in TIFF format]

Publication:

The molecular basis of tRNA selectivity by human pseudouridine synthase 3

Lin TY, Kleemann L, Jeżowski J, Dobosz D, Rawski M, Indyka P, Ważny G, Mehta R, Chramiec-Głąbik A, Koziej Ł, Ranff T, Fufezan C, Wawro M, Kochan J, Bereta J, Leidel SA, Glatt S

Molecular cell 84 (2024) 2472-2489.e8

PMID: 38996458

DOI: 10.1016/j.molcel.2024.06.013

Related EMDB entry:

EMD-16917

Deposited:

2023-03-28

Released:

2024-08-26

Last modified:

2024-08-26

Imageset size:

3.43 TB

Imageset DOI:

10.6019/EMPIAR-11510

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Human PUS3 apo structure

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

8211

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

high tension 300kV
spherical aberration 2.7mm
pixel size 0.86 A/px
total dose on vacuum 40.06 e/A2
frames per movie 40

defocus range set -1.5, -1.2, -0.9 um

sample tilt 0 deg

imaging mode EFTEM
magnification of hole image 11500x
magnification of exposure image 105000x
beam size 0.75 um
C2 aperture 50um
objective aperture retracted

Gatan BioQuantum energy filter
energy slit 20eV
Gatan K3 counting mode
dose rate on vacuum 16.35 e/px/s
exposure image resolution 5760 x 4092
physical pixel (Super Resolution off)
image type .tiff (gain corrected)

EPU version 2.10.0.1941REL

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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