EMPIAR-11308 - Cryo-EM structure of the folate-specific ECF transporter complex in MSP2N2 lipid nanodiscs bound to AMP-PNP (200 kV) [3527 multi-frame micrographs composed of 60 frames each in TIFF format]

EMPIAR-11308

Cryo-EM structure of the folate-specific ECF transporter complex in MSP2N2 lipid nanodiscs bound to AMP-PNP (200 kV) [3527 multi-frame micrographs composed of 60 frames each in TIFF format]

Publication:

Expulsion mechanism of the substrate-translocating subunit in ECF transporters

Thangaratnarajah C, Nijland M, Borges-Araújo L, Jeucken A, Rheinberger J, Marrink SJ, Souza PCT, Paulino C, Slotboom DJ

Nature communications 14 (2023)

PMID: 37491368

DOI: 10.1038/s41467-023-40266-1

Related EMDB entry:

EMD-16121

Deposited:

2022-11-15

Released:

2023-08-18

Last modified:

2023-08-18

Imageset size:

664.63 GB

Imageset DOI:

10.6019/EMPIAR-11308

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movie frames

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3527

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.022, 1.022)

Details:

Unaligned multi-frame movies with each containing 60 frames per movie collected from a Quantifoil Au 1.2/1.3 300 mesh grid at 200 kV in counting mode. Total accumulated electron exposure on the specimen level corresponds to 50.1 electrons per Å^2. Movies are not gain corrected and the gain reference is provided. Data acquired with SerialEM using a 3 x 3 multi-shot array.

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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