EMPIAR-11218 - cryoEM movies from 30S-RbfA complex [3369 multi-frame micrographs composed of 20 frames each in MRC format]

EMPIAR-11218

cryoEM movies from 30S-RbfA complex [3369 multi-frame micrographs composed of 20 frames each in MRC format]

Publication:

A conserved rRNA switch is central to decoding site maturation on the small ribosomal subunit.

Schedlbauer A, Iturrioz I, Ochoa-Lizarralde B, Diercks T, López-Alonso JP, Lavin JL, Kaminishi T, Çapuni R, Dhimole N, de Astigarraga E, Gil-Carton D, Fucini P, Connell SR

Science advances 7 (2021)

PMID: 34088665

DOI: 10.1126/sciadv.abf7547

Related EMDB entry:

EMD-12240 EMD-12243 EMD-12251 EMD-11753 EMD-12239 EMD-12241 EMD-12242 EMD-11751 EMD-11756

Deposited:

2021-03-31

Released:

2022-11-15

Last modified:

2022-11-15

Imageset size:

3.49 TB

Imageset DOI:

10.6019/EMPIAR-11218

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe movies of E. coli 30S subunit bound by RbfA

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

3369

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.05, 1.05)

Details:

K2 (counting) movies collected at eBIC - Titan Krios (M02, em15422) at 300 kV, 1.05 Å/pixel and a total exposure of 38.8 e-/Å^2

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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