EMPIAR-11213 - Mouse heavy chain apoferritin in vitreous ice after laser-melting and revitrification [12047 multi-frame micrographs composed of 553 frames each in EER format]

EMPIAR-11213

Mouse heavy chain apoferritin in vitreous ice after laser-melting and revitrification [12047 multi-frame micrographs composed of 553 frames each in EER format]

Publication:

Microsecond melting and revitrification of cryo samples with a correlative light-electron microscopy approach

Bongiovanni G, Harder OF, Drabbels M, Lorenz UJ

Frontiers in molecular biosciences 9 (2022)

PMID: 36438663

DOI: 10.3389/fmolb.2022.1044509

Related EMDB entry:

EMD-15620

Deposited:

2022-08-19

Released:

2023-08-18

Last modified:

2023-08-18

Imageset size:

2.18 TB

Imageset DOI:

10.6019/EMPIAR-11213

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of apoferritin in laser melted and revitrified ice

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

12047

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.455, 0.455)

Details:

The total dose is 50 electrons per squared Angstrom. Collected on a Titan Krios G4 at 300 kV acceleration voltage.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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