EMPIAR-11199 - Thermostabilized human prestin in complex with chloride, sulfate or salicylate [multiple data sets in TIFF format]

EMPIAR-11199

Thermostabilized human prestin in complex with chloride, sulfate or salicylate [multiple data sets in TIFF format]

Publication:

Cryo-EM structures of thermostabilized prestin provide mechanistic insights underlying outer hair cell electromotility

Futamata H, Fukuda M, Umeda R, Yamashita K, Tomita A, Takahashi S, Shikakura T, Hayashi S, Kusakizako T, Nishizawa T, Homma K, Nureki O

Nature communications 13 (2022)

PMID: 36266333

DOI: 10.1038/s41467-022-34017-x

Related EMDB entry:

EMD-31759 EMD-31757 EMD-31758

Deposited:

2022-06-28

Released:

2023-04-14

Last modified:

2023-04-14

Imageset size:

3.51 TB

Imageset DOI:

10.6019/EMPIAR-11199

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Cl bound

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

9361

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

HT: 300 kV
Cs: 2.7 mm
Flux: 0.84 e/Å2/frame

Please discard the first frame, which was corrupted due to a problem with the CDS mode.
Please use 'CountCDSRef_futamata1-3_3-A-001_0000_Feb22_11.46.30.mrc' as a gain reference without rotation or flipping.
Four digits (0000 to 0008) in the file names represent the direction of beam shift.

Files:

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Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

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Download a list of selected files.

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Sulfate bound

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

8155

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

HT: 300 kV
Cs: 2.7 mm
Flux: 0.84 e/Å2/frame

Please discard the first frame, which was corrupted due to a problem with the CDS mode.
Please use 'CountCDSRef_futamata4-1_36-A-001_0000_Mar05_13.25.01.mrc' as a gain reference without rotation or flipping.
Four digits (0000 to 0008) in the file names represent the direction of beam shift.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Salicylate bound

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6751

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

HT: 300 kV
Cs: 2.7 mm
Flux: 0.84 e/Å2/frame

Please discard the first frame, which was corrupted due to a problem with the CDS mode.
Please use 'CountCDSRef_futamata3-2_69-6_0000_Mar03_17.09.30.mrc' as a gain reference without rotation or flipping.
Four digits (0000 to 0008) in the file names represent the direction of beam shift.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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