EMPIAR-11094 - PMCA-amplified α-synuclein fibrils, Parkinson's Disease patient-derived seeds [6780 micrographs in MRC format]

EMPIAR-11094

PMCA-amplified α-synuclein fibrils, Parkinson's Disease patient-derived seeds [6780 micrographs in MRC format]

Publication:

Quaternary structure of patient-homogenate amplified α-synuclein fibrils modulates seeding of endogenous α-synuclein

Frieg B, Geraets JA, Strohäker T, Dienemann C, Mavroeidi P, Jung BC, Kim WS, Lee SJ, Xilouri M, Zweckstetter M, Schröder GF

(---)

Related EMDB entry:

EMD-13123

Deposited:

2021-06-29

Released:

2022-07-27

Last modified:

2022-07-27

Imageset size:

639.23 GB

Imageset DOI:

10.6019/EMPIAR-11094

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Aligned averages of PMCA-amplified α-synuclein fibrils, Parkinson's Disease patient-derived seeds

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

6780

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.05, 1.05)

Details:

Provided are aligned average micrographs collected on a Titan Krios at 300kV with a K3 detector operating in non-superresolution counting mode. Applied defocus values ranged from −0.7 to −2.9 µm. MotionCor2 was used to align 40 dose fractions that were taken with a total electron dose of ~42.7e-/Å². Coordinates provided are of start-end pairs, for helical segmentation and extraction.

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