EMPIAR-10916 - Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the frontal cortex of an individual with Alzheimer’s disease of amyloid-β 42 filaments [1762 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10916

Single particle cryo-EM dataset of sarkosyl-insoluble fraction from the frontal cortex of an individual with Alzheimer’s disease of amyloid-β 42 filaments [1762 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Cryo-EM structures of amyloid-β 42 filaments from human brains

Yang Y, Arseni D, Zhang WJ, Huang M, Lovestam SKA, Schweighauser M, Kotecha A, Murzin AG, Peak-Chew S, Macdonald J, Lavenir I, Garringer HJ, Gelpi E, Newell KL, Kovacs GG, Vidal R, Ghetti B, Falcon B, Scheres SHW, Goedert M

Science (New York, N.Y.) 375 (2022) 167-172

PMID: 35025654

DOI: 10.1126/science.abm7285

Related EMDB entry:

EMD-13800

Deposited:

2021-11-01

Released:

2022-01-25

Last modified:

2023-01-23

Imageset size:

531.91 GB

Imageset DOI:

10.6019/EMPIAR-10916

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1762

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.727, 0.727)

Details:

The files were written as eer files by EPU and then converted to TIFF files by relion_convert_to_tiff. During this process, the pixel size in the header was lost. The real pixel size is 0.727 A/px. The dose rate is 1 e/A2/frame. Please use gain-reference.mrc as a gain reference without any rotation or flipping.
pick_coordinates contains start-end coordinates of the manually picked filaments.
Refine097_run_data.star contains STAR file(s) with the metadata of the final refined segments.

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Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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