EMPIAR-10911 - Cryo-EM micrographs of GABA(A)Rs purified from cells expressing human full-length alpha4, beta3 and delta subunits, in presence of GABA, histamine, Ro15-4513 and nanobody Nb25 [10465 multi-frame micrographs composed of 48 frames each in TIFF format]

EMPIAR-10911

Cryo-EM micrographs of GABA(A)Rs purified from cells expressing human full-length alpha4, beta3 and delta subunits, in presence of GABA, histamine, Ro15-4513 and nanobody Nb25 [10465 multi-frame micrographs composed of 48 frames each in TIFF format]

Publication:

Differential assembly diversifies GABA_A receptor structures and signalling

Sente A, Desai R, Naydenova K, Malinauskas T, Jounaidi Y, Miehling J, Zhou X, Masiulis S, Hardwick SW, Chirgadze DY, Miller KW, Aricescu AR

Nature (2022)

PMID: 35355020

DOI: 10.1038/s41586-022-04517-3

Related EMDB entry:

EMD-14069 EMD-14071

Deposited:

2021-12-29

Released:

2022-04-04

Last modified:

2022-04-04

Imageset size:

2.82 TB

Imageset DOI:

10.6019/EMPIAR-10911

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of GABA(A)Rs purified from cells epxressing human full-length alpha4, beta3 and delta subunits, in presence of GABA, histamine, Ro15-4513 and nanobody Nb25

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

10465

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.326, 0.326)

Details:

K3 super-resolution movies with a total dose of 46.76 e/A2 and exposure time of 1.31 s.
The gain reference used for data processing is given in data/gain.mrc. No rotation or flipping is required.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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