EMPIAR-10790 - High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells [multiple data sets in TIFF and MRC formats]

EMPIAR-10790

High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells [multiple data sets in TIFF and MRC formats]

Publication:

High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells

Herdman M, von Kügelgen A, Kureisaite-Ciziene D, Duman R, El Omari K, Garman EF, Kjaer A, Kolokouris D, Löwe J, Wagner A, Stansfeld PJ, Bharat TAM

Structure (2021)

PMID: 34800371

DOI: 10.1016/j.str.2021.10.012

Related EMDB entry:

EMD-13355

Deposited:

2021-08-11

Released:

2022-01-18

Last modified:

2022-01-18

Imageset size:

304.36 GB

Imageset DOI:

10.6019/EMPIAR-10790

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Unaligned multiframe micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours (converted from .mrc into .tif with relion_convert_to_tiff)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

903

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.08, 1.08)

Details:

Unaligned multiframe micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours (converted from .mrc into .tif with relion_convert_to_tiff). The movies contain 20 frames accumulating 44.8 electrons per squared Angstrom in a 8 second exposure. The raw movies were collected using EPU and saved as non-gain corrected MRC files, which have been compressed with 'relion_convert_to_tif' before deposition (see https://relion.readthedocs.io/en/latest/Reference/MovieCompression.html).

Files:

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2. Unaligned multiframe micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours with a 30 degree stage tilt (converted from .mrc into .tif with relion_convert_to_tiff)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1115

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.08, 1.08)

Details:

Unaligned multiframe micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours with a 30 degree stage tilt (converted from .mrc into .tif with relion_convert_to_tiff). The movies contain 20 frames accumulating 44.8 electrons per squared Angstrom in a 8 second exposure. The raw movies were collected using EPU and saved as non-gain corrected MRC files, which have been compressed with 'relion_convert_to_tif' before deposition (see https://relion.readthedocs.io/en/latest/Reference/MovieCompression.html).

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Aligned and dose-weighted micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

903

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.08, 1.08)

Details:

Aligned and dose-weighted micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Aligned and dose-weighted micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours with a 30 degree stage tilt

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

1115

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.08, 1.08)

Details:

Aligned and dose-weighted micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours with a 30 degree stage tilt.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

5. Gain reference image of the non-tilted and tilted dataset in MRC format

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.08, 1.08)

Details:

Gain reference image of the non-tilted and tilted dataset in MRC format

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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