EMPIAR-10741 - Single particle cryo-EM dataset of Sars-CoV2 Rna dependent RNA polymerase [7043 multi-frame micrographs composed of 80 frames each in TIFF format]

EMPIAR-10741

Single particle cryo-EM dataset of Sars-CoV2 Rna dependent RNA polymerase [7043 multi-frame micrographs composed of 80 frames each in TIFF format]

Publication:

The structure of a dimeric form of SARS-CoV-2 polymerase

Jochheim FA, Tegunov D, Hillen HS, Schmitzová J, Kokic G, Dienemann C, Cramer P

Communications biology 4 (2021)

PMID: 34429502

DOI: 10.1038/s42003-021-02529-9

Related EMDB entry:

EMD-13116

Deposited:

2021-06-24

Released:

2021-10-25

Last modified:

2022-02-25

Imageset size:

2.72 TB

Imageset DOI:

10.6019/EMPIAR-10741

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned TIF movies of SARS-CoV2 RdRp in complex with nsp7, nsp8 and RNA

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7043

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.834, 0.834)

Details:

Movies are in the 2020-09-XX subfolders. They are from K3 (non-superres) and uncorrected (gain can be found in ./gain subfolders). Each LZW compressed.tif movie is accompanied with its respective .xml metadata file generated by Warp. Movies contain 80 frames collected over 2.2 seconds with a total dose of 60 e-/A2. Data was collected at 30 degree stage tilt. Particle locations were picked with Warp (locations are stored in ./matching subfolder and combined in the allparticles star file, extracted particles are in the ./particles). Micrograph thumbnails can be found in ./thumbnails subfolders.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

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