EMPIAR-10605 - Single particle cryo-EM dataset of Mus musculus mitochondrial complex I with ND6 P25L mutation [1492 multi-frame micrographs composed of 40 frames each in MRC format]

EMPIAR-10605

Single particle cryo-EM dataset of Mus musculus mitochondrial complex I with ND6 P25L mutation [1492 multi-frame micrographs composed of 40 frames each in MRC format]

Publication:

Structural basis for a complex I mutation that blocks pathological ROS production

Yin Z, Burger N, Kula-Alwar D, Aksentijević D, Bridges HR, Prag H, Grba D, Viscomi C, James AJ, Mottahedin A, Kreig T, Murphy MP, Hirst J

Nature communications 12 (2021)

PMID: 33514727

DOI: 10.1038/s41467-021-20942-w

Related EMDB entry:

EMD-11811

Deposited:

2020-12-10

Released:

2021-02-17

Last modified:

2021-02-17

Imageset size:

3.09 TB

Imageset DOI:

10.6019/EMPIAR-10605

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Single particle cryo-EM dataset of Mus musculus mitochondrial complex I with ND6 P25L mutation

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1492

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.054, 1.054)

Details:

Micrographs were imaged using a Gatan K2 summit camera in counting mode and GIF Quantum energy filter (20 eV slit width) on an FEI 300 keV Titan Krios microscope with a 100 μm objective aperture. 10s exposures captured in 40 frames and a total dose of 50 electrons Å-2. Double-exposed micrographs were not included in the deposited dataset.

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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