EMPIAR-10466 - Tilt-series from cryo-lamellae of WT S. cerevisiae cells [multiple data sets in MRC format]

EMPIAR-10466

Tilt-series from cryo-lamellae of WT S. cerevisiae cells [multiple data sets in MRC format]

Publication:

In-cell architecture of the nuclear pore and snapshots of its turnover

Allegretti M, Zimmerli CE, Rantos V, Wilfling F, Ronchi P, Fung HKH, Lee CW, Hagen W, Turoňová B, Karius K, Börmel M, Zhang X, Müller CW, Schwab Y, Mahamid J, Pfander B, Kosinski J, Beck M

Nature (2020)

PMID: 32879490

DOI: 10.1038/s41586-020-2670-5

Related EMDB entry:

EMD-10198

Deposited:

2020-06-03

Released:

2020-10-16

Last modified:

2020-10-16

Imageset size:

605.75 GB

Imageset DOI:

10.6019/EMPIAR-10466

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Raw tilt series from S. cerevisiae WT cells containing nuclear pore complexes (each tilt is an aligned average of ~15 frames by serial em)

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

177

Image size:

(3708, 3838)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(None, None)

Details:

The number of tilts varies between tilt-series

Files:

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Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Tilt series of WT cerevisiae cells after cleaning bad tilts and dose filtering

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

177

Image size:

(3708, 3708)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(None, None)

Details:

The number of tilts varies between tilt-series

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

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Citations

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Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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