EMPIAR-10465 - Single-particle cryo-EM dataset of the complex between a nucleosome and the regulatory linker of ALC1 [multiple data sets in TIFF and MRCS formats]

EMPIAR-10465

Single-particle cryo-EM dataset of the complex between a nucleosome and the regulatory linker of ALC1 [multiple data sets in TIFF and MRCS formats]

Publication:

Mechanistic Insights into Regulation of the ALC1 Remodeler by the Nucleosome Acidic Patch

Lehman LC, Bacic L, Hewitt G, Brackmann K, Sabantsev A, Gaullier G, Pytharopoulou S, Degliesposti G, Okkenhaug H, Tan S, Costa A, Skehel JM, Boulton SJ, Deindl S

Cell reports 33 (2020)

PMID: 33357431

DOI: 10.1016/j.celrep.2020.108529

Related EMDB entry:

EMD-11220 EMD-11221

Deposited:

2020-06-26

Released:

2021-01-29

Last modified:

2021-01-29

Imageset size:

6.84 TB

Imageset DOI:

10.6019/EMPIAR-10465

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - multiframe - processed

Image Sets

1. Unaligned movies of the nucleosome - ALC1-linker complex - session_1_disk_1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2852

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.654, 0.654)

Details:

60 gain-corrected frames per movie, collected on Titan Krios with K3 in counting mode.

Files:

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2. Unaligned movies of the nucleosome - ALC1-linker complex - session_1_disk_2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5984

Image size:

(5760, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.654, 0.654)

Details:

60 gain-corrected frames per movie, collected on Titan Krios with K3 in counting mode.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Unaligned movies of the nucleosome - ALC1-linker complex - session_1_disk_3

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2287

Image size:

(5760, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.654, 0.654)

Details:

60 gain-corrected frames per movie, collected on Titan Krios with K3 in counting mode.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Unaligned movies of the nucleosome - ALC1-linker complex - session_2_grid_1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6155

Image size:

(5760, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.654, 0.654)

Details:

60 gain-corrected frames per movie, collected on Titan Krios with K3 in counting mode.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

5. Unaligned movies of the nucleosome - ALC1-linker complex - session_2_grid_2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2619

Image size:

(5760, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.654, 0.654)

Details:

60 gain-corrected frames per movie, collected on Titan Krios with K3 in counting mode.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

6. Particles of the high-resolution nucleosome class

Category:

picked particles - multiframe - processed

Image format:

MRCS

No. of images or tilt series:

636505

Image size:

(300, 300)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.654, 0.654)

Details:

Particles from final Refine3D job in RELION-3.1-5857e1 for the high-resolution nucleosome class.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

7. Particles of the hexasome class

Category:

picked particles - multiframe - processed

Image format:

MRCS

No. of images or tilt series:

414605

Image size:

(300, 300)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.654, 0.654)

Details:

Particles from final Refine3D job in RELION-3.1-5857e1 for the hexasome class.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

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Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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