EMPIAR-10453 - Cryo electron tomography - tilt-series of Sars-Cov-2 [266 tilt series in MRC format]

EMPIAR-10453

Cryo electron tomography - tilt-series of Sars-Cov-2 [266 tilt series in MRC format]

Publication:

In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges

Turoňová B, Sikora M, Schürmann C, Hagen WJH, Welsch S, Blanc FEC, von Bülow S, Gecht M, Bagola K, Hörner C, van Zandbergen G, Landry J, de Azevedo NTD, Mosalaganti S, Schwarz A, Covino R, Mühlebach MD, Hummer G, Krijnse Locker J, Beck M

Science (New York, N.Y.) 370 (2020) 203-208

PMID: 32817270

DOI: 10.1126/science.abd5223

Related EMDB entry:

EMD-11223 EMD-11222 EMD-11347

Deposited:

2020-06-25

Released:

2020-07-10

Last modified:

2021-11-02

Imageset size:

504.45 GB

Imageset DOI:

10.6019/EMPIAR-10453

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Unaligned tilt-series of Sars-Cov-2

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

266

Image size:

(5760, 4092)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.329, 1.329)

Details:

Tilt-series are sorted according to ascending tilt angle apart from tilt-series 200-237 which are in order of acquisition (the dose-symmetric scheme with tilt range -60 to 60 degrees and 3 degree step was used).

Mdoc files corresponding to the tilt-series are also provided.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

See all citations

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