EMPIAR-10444 - Cryo-EM structures of calcium homeostasis modulator (CALHM) channels [multiple data sets in TIFF format]

EMPIAR-10444

Cryo-EM structures of calcium homeostasis modulator (CALHM) channels [multiple data sets in TIFF format]

Publication:

Cryo-EM structures of calcium homeostasis modulator channels in diverse oligomeric assemblies

Demura K, Kusakizako T, Shihoya W, Hiraizumi M, Nomura K, Shimada H, Yamashita K, Nishizawa T, Taruno A, Nureki O

Science Advances 6 (2020)

DOI: 10.1126/sciadv.aba8105

Related EMDB entry:

EMD-0922 EMD-0920 EMD-0923 EMD-0921 EMD-0919

Deposited:

2020-06-10

Released:

2020-07-30

Last modified:

2020-07-30

Imageset size:

6.76 TB

Imageset DOI:

10.6019/EMPIAR-10444

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movies for OlCALHM1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3075

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

Gain reference is provided, which need to be flipped ('Flip upside down (1)' in RELION).

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Unaligned movies for HsCALHM2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2110

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.8346, 0.8346)

Details:

Unaligned, gain corrected movies from Falcon 3 in the counting mode. The MRC files from EPU were converted to the TIFF format by mrc2tif in IMOD. During this process, the pixel size in the image header became 1.0. Note that we started processing with the nominal pixel size of 0.861 Å and finally changed the value in PostProcess so that the pixels had the calibrated size of 0.8346 Å.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Unaligned movies for CeCLHM-1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2906

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.8346, 0.8346)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Unaligned movies for OlCALHM1-HsCALHM2 chimera

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2808

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

Gain reference is provided, which need to be flipped ('Flip upside down (1)' in RELION).

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

Protein Data Bank Japan News

[2024-11-20]

224 new PDB entries have been released on 2024-11-20.

[2024-11-01]

A new portal integrating genome variants and protein structures has released

[2024-10-30]

[wwPDB] Deprecation of FTP File Download Protocol on November 1, 2024

[2024-10-25]

PDBj luncheon seminar at APBJC 2024

[2024-10-17]

[wwPDB] The Nobel Prize in Chemistry 2024