EMPIAR-10393 - Subnanometer-resolution structure determination in situ by a hybrid subtomogram averaging - single particle cryoEM - workflow

EMPIAR-10393

Subnanometer-resolution structure determination in situ by a hybrid subtomogram averaging - single particle cryoEM - workflow - on TMV [4 tilt series in MRC format]

Publication:

Subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM

Sanchez RM, Zhang Y, Chen W, Dietrich L, Kudryashev M

Nature communications 11 (2020)

PMID: 32709843

DOI: 10.1038/s41467-020-17466-0

Related EMDB entry:

EMD-10834

Deposited:

2020-04-06

Released:

2020-07-06

Last modified:

2020-08-25

Imageset size:

37.49 GB

Imageset DOI:

10.6019/EMPIAR-10393

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. TMV-hybrid tomography- tiltstacks- aligned and CTF corrected

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(7420, 7676)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.1, 1.1)

Details:

TMV testing data - hybrid tiltstacks - motioncor2 aligned and CTF corrected
Subtomogram averaging table - sta_rslt.tbl
Script for creating refine particle stack from StA table - create_stack_b1.m
Refine particle stack - hyb_b1.mrcs
Refine particle star file - hyb_b1.star
Refine helical parameter - refine_command_b1.txt

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