EMPIAR-10340 - Cryo-EM reconstruction of tau filaments extracted from the brains of three individuals with Corticobasal degeneration [multiple data sets in TIFF format]

EMPIAR-10340

Cryo-EM reconstruction of tau filaments extracted from the brains of three individuals with Corticobasal degeneration [multiple data sets in TIFF format]

Publication:

Novel tau filament fold in corticobasal degeneration

Zhang W, Tarutani A, Newell KL, Murzin AG, Matsubara T, Falcon B, Vidal R, Garringer HJ, Shi Y, Ikeuchi T, Murayama S, Ghetti B, Hasegawa M, Goedert M, Scheres SHW

Nature (2020)

PMID: 32050258

DOI: 10.1038/s41586-020-2043-0

Related EMDB entry:

EMD-10514 EMD-10512

Deposited:

2019-11-26

Released:

2020-03-02

Last modified:

2020-03-02

Imageset size:

3.04 TB

Imageset DOI:

10.6019/EMPIAR-10340

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movies for Case 1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2567

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.15, 1.15)

Details:

Movies/Case1 contains unaligned movies for Case1. The files were written as gain-normalised floating point MRC files by EPU and then converted into gain-unnormalised floating point TIFF files by relion_convert_to_tiff. During this process, the pixel size in the header was lost. The real pixel size is 1.15 A/px. The dose rate is 1.346 e/A2/frame. Please use Movies/Case1/gain-reference.mrc as a gain reference without any rotation or flipping.

Micrographs/Case1 contains aligned and summed micrographs.

Coordinates/Case1 contains manually picked filament coordinates for Type I and Type II filaments before any classifications.

Particles/Case1 contains polished "shiny" particles used for final reconstructions.

Files:

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Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Unaligned movies for Case 2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4442

Image size:

(3838, 3710)

Pixel type:

SIGNED BYTE

Pixel spacing:

(1.15, 1.15)

Details:

Movies/Case2 contains unaligned movies for Case2. The files were written as gain-unnormalised integer MRC files by EPU and then converted to TIFF files by mrc2tif in the IMOD suite. During this process, the pixel size in the header was lost. The real pixel size is 1.15 A/px. The dose rates are 1.1 and 2.0 e/A2/frame for low-dose and high-dose datasets, respectively. Please use Movies/Case2/Movie-lowdose/gain.mrc and Movies/Case2/Movie-highdose/gain.mrc, respectively, as gain references without any rotation or flipping.

Micrographs/Case2 contains aligned and summed micrographs.

Coordinates/Case2 contains manually picked filament coordinates for Type I and Type II filaments before any classifications.

Particles/Case2 contains polished "shiny" particles used for final reconstructions.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Unaligned movies for Case 3

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3114

Image size:

(3838, 3710)

Pixel type:

SIGNED BYTE

Pixel spacing:

(1.15, 1.15)

Details:

Movies/Case3 contains unaligned movies for Case3. The files were written as gain-unnormalised integer MRC files by EPU and then converted to TIFF files by mrc2tif in the IMOD suite. During this process, the pixel size in the header was lost. The real pixel size is 1.15 A/px. The dose rate is 1.094 e/A2/frame. Please use Movies/Case3/gain.mrc as a gain reference without any rotation or flipping.

Micrographs/Case3 contains aligned and summed micrographs.

Coordinates/Case3 contains manually picked filament coordinates for Type I and Type II filaments before any classifications.

Particles/Case3 contains polished "shiny" particles used for final reconstructions.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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