EMPIAR-10325
Raw FIB SEM images of a parasitophorous vacuole containing Toxoplasma gondii ∆CAP parasites, complemented with CAP. [4529 micrographs in TIFF format]
Publication:

Differential requirements for cyclase-associated protein (CAP) in actin-dependent processes of Toxoplasma gondii

Hunt A, Russell MRG, Wagener J, Kent R, Carmeille R, Peddie CJ, Collinson L, Heaslip A, Ward GE, Treeck M

eLife 8 (2019)

PMID: 31577230

Related EMDB entry:
Deposited:
2019-09-30
Released:
2019-10-07
Last modified:
2019-10-07
Imageset size:
605.18 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - single frame
1. Raw FIB SEM images of a parasitophorous vacuole containing Toxoplasma gondii ∆CAP parasites, complemented with CAP.
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
4529
Image size:
(8384, 8384)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(50.0, 50.0)
Details:
Image acquisition;
5 nm voxels: xy pixel set to 5 nm, FIB mill every 5 nm.
SEM: 1.5 kV with 1 nA current, 10 μs dwell time.
EsB detector grid voltage: 1200 V.
Ion beam milling: 30 kV, 700 pA.
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