EMPIAR-10313 - Cryo-EM reconstructions of tau filaments in chronic traumatic encephalopathy [multiple data sets in MRCS and MRC formats]

EMPIAR-10313

Cryo-EM reconstructions of tau filaments in chronic traumatic encephalopathy [multiple data sets in MRCS and MRC formats]

Publication:

Novel tau filament fold in chronic traumatic encephalopathy encloses hydrophobic molecules

Falcon B, Zivanov J, Zhang W, Murzin AG, Garringer HJ, Vidal R, Crowther RA, Newell KL, Ghetti B, Goedert M, Scheres SHW

Nature 568 (2019) 420-423

PMID: 30894745

DOI: 10.1038/s41586-019-1026-5

Related EMDB entry:

EMD-0528 EMD-0527

Deposited:

2019-08-20

Released:

2020-02-07

Last modified:

2020-02-07

Imageset size:

1.24 TB

Imageset DOI:

10.6019/EMPIAR-10313

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies (Movies/*.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Compression was carried out by separating (“un-normalizing”) the gain.
The details of the method are described in
https://relion.readthedocs.io/en/release-3.1/Reference/MovieCompression.html#gain-normalised-movies.

Please apply gain-reference.mrc during processing.

Original size:  10,161,964,824 kilobytes (4060 movies)
Compressed size: 1,037,337,028 kilobytes

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Raw Movies

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

5420

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.055, 1.055)

Details:

The dose rate is 1.23 electrons / A^2 / frame.

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2. Aligned Micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

5420

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.055, 1.055)

Details:

Aligned and dose-weighted sums. Particle positions are in the particles.star files.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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