EMPIAR-10157 - Cryo electron tomography of sea urchin sperm flagella [31 tilt series in MRC format]

EMPIAR-10157

Cryo electron tomography of sea urchin sperm flagella [31 tilt series in MRC format]

Publication:

Asymmetric distribution and spatial switching of dynein activity generates ciliary motility

Lin J, Nicastro D

Science (New York, N.Y.) 360 (2018)

PMID: 29700238

DOI: 10.1126/science.aar1968

Related EMDB entry:

EMD-8838 EMD-8836

Deposited:

2018-02-14

Released:

2018-04-27

Last modified:

2021-05-28

Imageset size:

15.80 GB

Imageset DOI:

10.6019/EMPIAR-10157

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Tilt series of sea urchin sperm flagella that were frozen while they were actively beating.

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(2048, 2048)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(9.856, 9.856)

Details:

These 31 tilt series were collected using a FEI Tecnai F30 electron microscope. Two sub-tomogram averages derived from these tilt series have been deposited in EMDB under ID codes EMD-8836 and EMD-8838.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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