EMPIAR-10089 - Single Particle cryoEM Dataset of TcdA1 in prepore state (SPHIRE tutorial) [97 multi-frame micrographs composed of 24 frames each in MRCS format]

EMPIAR-10089

Single Particle cryoEM Dataset of TcdA1 in prepore state (SPHIRE tutorial) [97 multi-frame micrographs composed of 24 frames each in MRCS format]

Publication:

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

Moriya T, Saur M, Stabrin M, Merino F, Voicu H, Huang Z, Penczek P, Raunser S, Gatsogiannis C

Journal of Visualised Experiments (---)

Related EMDB entry:

EMD-3645

Deposited:

2017-03-21

Released:

2017-05-24

Last modified:

2017-05-24

Imageset size:

72.76 GB

Imageset DOI:

10.6019/EMPIAR-10089

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of TcdA1 in prepore state (SPHIRE tutorial dataset)

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.14, 1.14)

Details:

The micrographs contain the unaligned raw movie frames (24 frames/micrograph). The corresponding .box files, contain the particle XY positions in EMAN1 format.

Files:

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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