EMPIAR-12731 - Structural mechanism of FusB-mediated rescue from fusidic acid inhibition of protein synthesis [multiple data sets in TIFF and EER formats]

EMPIAR-12731

Structural mechanism of FusB-mediated rescue from fusidic acid inhibition of protein synthesis [multiple data sets in TIFF and EER formats]

Publication:

Structural mechanism of FusB-mediated rescue from fusidic acid inhibition of protein synthesis

González-López A, Ge X, Larsson DSD, Sihlbom Wallem C, Sanyal S, Selmer M

Nature communications 16 (2025)

PMID: 40251147

DOI: 10.1038/s41467-025-58902-3

Related EMDB entry:

EMD-51352 EMD-51351 EMD-51353 EMD-51354 EMD-51355 EMD-51356 EMD-51357 EMD-51350

Deposited:

2025-04-14

Released:

2025-05-17

Last modified:

2025-05-17

Imageset size:

28.19 TB

Imageset DOI:

10.6019/EMPIAR-12731

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Data for preliminary dataset1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3490

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.952, 0.952)

Details:

Collected on a Glacios TEM operated at 200 kV equipped with a Falcon-III direct electron detector. The data were acquired at ×150,000 nominal magnification with a pixel size of 0.952 Å. A total of 3490 movies were collected in 30 frames with a total dose of 30.97 e−/Å2 (1.01 e−/pixel/s) over 27.79 s with a set defocus between−0.6 and−1.4 µm.
The movies are gain corrected.
All the 70S particles (reference based motion corrected) are provided, as well as star files and maps for the relevant reconstructions.

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2. Data for preliminary dataset2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

8533

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.824, 0.824)

Details:

Collected on a Titan Krios G2 operated at 300 kV and equipped with a K3 BioQuantum direct electron detector and energy filter using 20 eV slit. The data were acquired at ×105,000 nominal magnification with a calibrated pixel size of 0.8240 Å. A total of 8533 movies were collected in 30 frames with a total dose of 28.52 e−/Å2 (16.1 e−/pixel/s) over 1 s with a set defocus between−0.8 and−1.4 µm.
The movies are gain corrected.
All the 70S particles (reference based motion corrected) are provided, as well as star files and maps for the relevant reconstructions.

Files:

This entry cannot be downloaded from PDBj.

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3. Data for the Early (6s) dataset

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

24479

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.648, 0.648)

Details:

Collected on a Titan Krios G3i operated at 300 kV and equipped with a K3 Bio-Quantum direct electron detector and energy lter using 20 eV slit. The data were acquired at ×130,000 nominal magnification with a calibrated pixel size of 0.648 Å. A total of 24,479 movies were collected in 40 frames with a total dose of 40 e−/Å2 (16.8 e−/pixel/s) over 1 s with a set defocus between−0.7 and−1.0 µm.
The movies are gain corrected.
All the 70S particles (reference based motion corrected) are provided, as well as star files and maps for the relevant reconstructions.

Files:

This entry cannot be downloaded from PDBj.

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4. Data for the 25 s dataset

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

32937

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.6478, 0.6478)

Details:

Collected on a Titan Krios operated at 300 kV and equipped with a K3 BioQuantum direct electron detector and energy filter using 20 eV slit. The data were acquired at ×130,000 nominal magnification with a calibrated pixel size of 0.6478 Å. A total of 32,937 were collected in 45 frames with a total dose of 45.47 e−/Å2 (15.9 e−/pixel/s) over 1.2 s with a set defocus between−0.7 and−1.0 µm.
The movies are not gain corrected. The gain file is provided.
All the 70S particles (reference based motion corrected) are provided, as well as star files and maps for the relevant reconstructions.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

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5. Data for the FusB-Sa70S dataset

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

10065

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.728, 0.728)

Details:

Collected on a Titan Krios G2 operated at 300 kV and equipped with a Falcon-4i direct electron detector and Selectris energy filter using 10 eV slit. The data were acquired at ×165,000 nominal magnification with a calibrated pixel size of 0.728 Å. A total of 10,065 EER formatted exposures were collected in 657 raw frames with a total dose of 28.14 e−/Å2 (14.06 e−/pixel/s) over 2.14 s with a set defocus between −0.7 and−1.3 µm.
The movies are not gain corrected. The gain file is provided.
All the 70S particles (reference based motion corrected) are provided, as well as star files and maps for the relevant reconstructions.

Files:

This entry cannot be downloaded from PDBj.

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We recommend using rsync, aspera, globus, etc.

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6. Screening data for the Early (6s) sample

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1318

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.7463, 0.7463)

Details:

Collected on a Glacios TEM operated at 200 kV equipped with a Falcon-III direct electron detector. The data were acquired at ×190,000 nominal magnification with a pixel size of 0.7463 Å. A total of 1318 movies were collected in 45 frames with a total dose of 42.91 e−/Å2 (0.86 e−/pixel/s) over 27.79 s with a set defocus between−0.5 and−1.5 µm.
The movies are gain corrected.

Files:

This entry cannot be downloaded from PDBj.

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7. Screening data for the 25 s sample

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5489

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.7463, 0.7463)

Details:

Collected on a Glacios TEM operated at 200 kV equipped with a Falcon-III direct electron detector. The data were acquired at ×190,000 nominal magnification with a pixel size of 0.7463 Å. A total of 5489 movies were collected in 46 frames with a total dose of 48.40 e−/Å2 (1.00 e−/pixel/s) over 28.41 s with a set defocus between−0.5 and−1.5 µm.
The movies are gain corrected.

Files:

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8. Screening data for the FusB-Sa70S dataset

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4393

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.952, 0.952)

Details:

Collected on a Glacios TEM operated at 200 kV equipped with a Falcon-III direct electron detector. The data were acquired at ×150,000 nominal magnification with a pixel size of 0.952 Å. A total of 4393 movies were collected in 31 frames with a total dose of 29.8 e−/Å2 (0.94 e−/pixel/s) over 28.74 s with a set defocus between−0.5 and−1.5 µm.
The movies are gain corrected.

Files:

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