EMPIAR-12695 - CryoEM of purified immature tick borne encephalitis virus particles, strain Kuutsalo-14 [multiple data sets in TIFF format]

EMPIAR-12695

CryoEM of purified immature tick borne encephalitis virus particles, strain Kuutsalo-14 [multiple data sets in TIFF format]

Publication:

The structure of immature tick-borne encephalitis virus supports the collapse model of flavivirus maturation

Anastasina M, Füzik T, Domanska A, Pulkkinen LIA, Šmerdová L, Formanová PP, Straková P, Nováček J, Růžek D, Plevka P, Butcher SJ

Science advances 10 (2024) eadl1888-eadl1888

PMID: 38959313

DOI: 10.1126/sciadv.adl1888

Related EMDB entry:

EMD-17809 EMD-17808

Deposited:

2024-08-02

Released:

2025-05-27

Last modified:

2025-05-27

Imageset size:

29.32 TB

Imageset DOI:

10.6019/EMPIAR-12695

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of immature TBEV (Kuutsalo-14) stored in *.tar.gz archives, data set 1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

26990

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.1041, 1.1041)

Details:

Microscope Details:
Accelerating voltage: 300 kV
Spherical aberration: 2.7 mm

Data acquisition details:
Nominal Magnification: 81,000X
Pixel Size: 1.1041Å
Dose Rate: 16 e/px/s
Exposure: 3.3s (247/75)
Total Dose: ~43e/Å²
Number of Frames: 50
Target Defocuses: -0.7um to -2.7um at 0.2um step
File format: Gain Normalized LZW compressed TIFF

Files:

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2. Unaligned multi-frame micrographs of immature TBEV (Kuutsalo-14) stored in *.tar.gz archives, data set 2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

24271

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.0859, 1.0859)

Details:

Microscope Details:
Accelerating voltage: 300 kV
Spherical aberration: 2.7 mm

Data acquisition details:
Nominal Magnification: 81000 X
Defocus Range: -0.6 um to -2.0 um
Defocus Step: 0.2 pm
Dose Rate: 19.514 e/px/s
Dose Rate: 16.549 e/square angstrom⁄s
Exposure Time: 2.6 s
Total Dose: 43.027 e/A2
Dose Fractions (type): 50 (Physical Pixel TIFF LZW Gain normalized)
Dose per Fraction: 0.861 e/square angstrom

Software: EPU v2.12.1
Selection Type: Automated
Acquisition Mode: Faster Acquisition

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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