EMPIAR-12613 - Cryo-EM structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys [multiple data sets in TIFF and MRC formats]

EMPIAR-12613

Cryo-EM structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys [multiple data sets in TIFF and MRC formats]

Publication:

Mechanism of release factor-mediated peptidyl-tRNA hydrolysis on the ribosome

Aleksandrova EV, Syroegin EA, Basu RS, Vassilevski AA, Gagnon MG, Polikanov YS

Science (New York, N.Y.) 388 (2025)

PMID: 40536958

DOI: 10.1126/science.ads9030

Related EMDB entry:

EMD-49594

Deposited:

2025-02-28

Released:

2025-07-12

Last modified:

2025-07-12

Imageset size:

4.12 TB

Imageset DOI:

10.6019/EMPIAR-12613

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Unaligned multiframe micrographs of Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

25104

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.8423, 0.8423)

Details:

---

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2. Gain reference image for uploaded unaligned micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.8423, 0.8423)

Details:

Gain reference file for the accompanying raw micrographs

Files:

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Download a list of selected files.

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Citations

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Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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