EMPIAR-12471 - DDM extracted overexpressed HflK/C nautilus-like assembly [16055 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-12471

DDM extracted overexpressed HflK/C nautilus-like assembly [16055 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

An asymmetric nautilus-like HflK/C assembly controls FtsH proteolysis of membrane proteins

Ghanbarpour A, Telusma B, Powell BM, Zhang JJ, Bolstad I, Vargas C, Keller S, Baker T, Sauer RT, Davis JH

bioRxiv NA (2024) NA-NA

PMID: NA

DOI: 10.1101/2024.08.09.604662

Related EMDB entry:

EMD-46062

Deposited:

2024-10-20

Released:

2025-01-09

Last modified:

2025-01-09

Imageset size:

2.10 TB

Imageset DOI:

10.6019/EMPIAR-12471

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned Movies of Overexpressed HflK/C complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

16055

Image size:

(4092, 5760)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.654, 0.654)

Details:

Nominal Defocus range (min, max, step): -0.5 to -2
Total exposure on specimen: 47.2 el/A2
Nominal pixel size: 0.654 A (prebinned by a factor of 2)
Objective lens spherical aberration (Cs): 2.7 mm
Acceleration voltage: 300 kV
Microscope / Detector: Krios G3i / K3

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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