EMPIAR-12460 - Cryo-ET tilt series of FIB-milled wild-type mouse embryonic stem cells [161 tilt series in MRC format]

EMPIAR-12460

Cryo-ET tilt series of FIB-milled wild-type mouse embryonic stem cells [161 tilt series in MRC format]

Publication:

Nuclear pores safeguard the integrity of the nuclear envelop

Taniguchi R, Orniacki C, Kreysing JP, Zila V, Zimmerli CE, Böhm S, Turoňová B, Kräusslich H-G, Doye V, Beck M

(---)

Related EMDB entry:

EMD-52153 EMD-52154 EMD-52155

Deposited:

2024-11-19

Released:

2025-01-29

Last modified:

2025-01-29

Imageset size:

401.23 GB

Imageset DOI:

10.6019/EMPIAR-12460

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Cryo-ET tilt series of FIB-milled wild-type mouse embryonic stem cells

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

161

Image size:

(5760, 4092)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(2.682, 2.682)

Details:

Images were recorded as movies of 10 frames and were motion-corrected on-the-fly in SerialEM. Tilt series were acquired from the stage pretilt angle of 8 degree, and images were collected using a dose-symmetric acquisition scheme with 2 degree increments, up to +64 or 66 and -52 degrees.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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