EMPIAR-12319 - CryoEM of E. coli RNA polymerase on T7A1 promoter DNA [6790 multi-frame micrographs composed of 30 frames each in TIFF format]

EMPIAR-12319

CryoEM of E. coli RNA polymerase on T7A1 promoter DNA [6790 multi-frame micrographs composed of 30 frames each in TIFF format]

Publication:

Structural origins of Escherichia coli RNA polymerase open promoter complex stability

Saecker RM, Chen J, Chiu CE, Malone B, Sotiris J, Ebrahim M, Yen LY, Eng ET, Darst SA

Proceedings of the National Academy of Sciences of the United States of America 118 (2021)

PMID: 34599106

DOI: 10.1073/pnas.2112877118

Related EMDB entry:

EMD-23897

Deposited:

2024-10-03

Released:

2024-12-21

Last modified:

2024-12-21

Imageset size:

1.57 TB

Imageset DOI:

10.6019/EMPIAR-12319

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned raw movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6790

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.06, 1.06)

Details:

FEI Titan Krios + Gatan K2, super-resolution, energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 7.24 e-/ A^2/s; pixel size 1.06 A/px(detector); pixel size 0.53 A/px(image); exposure 6 s; frame time 0.2 s; total dose 43.45 e-/A^2; total frames 30; defocus range -1.1 to -2.4 um; gain reference file 18nov08d_08110340_01_7676x7420_norm_0_mod.mrc is in references folder

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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