EMPIAR-12292 - Cryo-electron tomography of optogenetically-induced lamellipodia [multiple data sets in MRC format]

EMPIAR-12292

Cryo-electron tomography of optogenetically-induced lamellipodia [multiple data sets in MRC format]

Publication:

Cryo-electron tomography of the actin cytoskeleton and membrane organization during lamellipodia formation using optogenetics

Inaba H, Imasaki T, Aoyama K, Yoshihara S, Takazaki H, Kato T, Goto H, Mitsuoka K, Nitta R, Nakata T

bioRxiv (2024)

DOI: 10.1101/2024.08.13.607852

Related EMDB entry:

EMD-61088 EMD-61091 EMD-61092 EMD-61093

Deposited:

2024-08-30

Released:

2025-03-19

Last modified:

2025-03-19

Imageset size:

57.82 GB

Imageset DOI:

10.6019/EMPIAR-12292

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

reconstructed volumes
tilt series

Image Sets

1. Final models

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(None, None)

Details:

- Titan Krios
- 300 kV
- 5 μm Cs corrected (CEOS, GmbH)
- BioQuantam K3 direct ditector, energy filter (slit width of 20 eV)
- 1.44 e/A2/frame
- no gain reference file

- EMD-61088：Cell1_Position1
- EMD-61091：Cell1_Position4
- EMD-61092：Cell2_Position1
- EMD-61093：Cell2_Position2

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2. Tilt series

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(5760, 4092)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(None, None)

Details:

---

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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