EMPIAR-12290 - Multi-frame micrographs of a 74 kDa Ang2-5A12.6DS Fab complex in tiff format. [10063 multi-frame micrographs composed of 119 frames each in TIFF format]

EMPIAR-12290

Multi-frame micrographs of a 74 kDa Ang2-5A12.6DS Fab complex in tiff format. [10063 multi-frame micrographs composed of 119 frames each in TIFF format]

Publication:

Disulfide constrained Fabs overcome target size limitation for high-resolution single-particle cryo-EM

Kung JE, Johnson MC, Jao CC, Arthur CP, Tegunov D, Rohou A, Sudhamsu J

bioRxiv (2024)

DOI: 10.1101/2024.05.10.593593

Related EMDB entry:

EMD-43220

Deposited:

2024-05-16

Released:

2025-07-14

Last modified:

2025-07-14

Imageset size:

4.26 TB

Imageset DOI:

10.6019/EMPIAR-12290

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Multi-frame micrographs of a 74 kDa Ang2-5A12.6DS Fab complex in tiff format.

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

10063

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.838, 0.838)

Details:

Multi-frame micrographs of a 74 kDa Ang2-5A12.6DS Fab complex in tiff format.
Gain file: CountCDSRef_movie_87-1_000_Dec05_16.39.19.mrc
Pixel spacing: 0.838Å
Total dose per image: 68.516 electrons/Å²
Frames per image: 119
Objective aperture diameter: 100μm
C2 aperture diameter: 50μm
Gun lens setting: 4
Energy filter slit width: 20eV

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

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Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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