EMPIAR-12108 - Cryogenic electron microscopy of blot-plunged acetyl-CoA decarbonylase/synthase [14078 multi-frame micrographs composed of 21 frames each in TIFF format]

EMPIAR-12108

Cryogenic electron microscopy of blot-plunged acetyl-CoA decarbonylase/synthase [14078 multi-frame micrographs composed of 21 frames each in TIFF format]

Publication:

Capturing a methanogenic carbon monoxide dehydrogenase/acetyl-CoA synthase complex via cryogenic electron microscopy

Biester A, Grahame DA, Drennan CL

Proceedings of the National Academy of Sciences of the United States of America 121 (2024)

PMID: 39361653

DOI: 10.1073/pnas.2410995121

Related EMDB entry:

EMD-45089

Deposited:

2024-05-28

Released:

2025-01-23

Last modified:

2025-01-23

Imageset size:

1.97 TB

Imageset DOI:

10.6019/EMPIAR-12108

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of acetyl-CoA decarbonylase/synthase (ACDS) complex and subcomplexes

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

14078

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.832, 0.832)

Details:

Gain reference:
data/GainsBackup2021-12-10/K3-18030019 Gain Ref.x1.m1.bin2.mrc
Defocus range: -0.8 to -2.1 µM
Total exposure on specimen: 54.53 el/A2
Exposure time: 1.69 s
Calibrated pixel size: 0.832 (0.416 A super res)
Objective lens spherical aberration (Cs): 2.7 mm
Acceleration voltage: 300 kV
Extraction voltage: 3550 V
Microscope / Detector: Krios G3i / K3

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Citations

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Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

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CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

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Few-shot learning for non-vitrified ice segmentation.

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Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

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Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

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