EMPIAR-11884 - E.coli DNA gyrase in complex with 217 bp substrate DNA and LEI-800 [17017 multi-frame micrographs composed of 639 frames each in EER format]

EMPIAR-11884

E.coli DNA gyrase in complex with 217 bp substrate DNA and LEI-800 [17017 multi-frame micrographs composed of 639 frames each in EER format]

Publication:

Discovery of isoquinoline sulfonamides as allosteric gyrase inhibitors with activity against fluoroquinolone-resistant bacteria

Bakker A.T., Kotsogianni I, Avalos M., Punt J.M., Liu B., Piermarini D., Gagestein B., Slingerland C.J., Zhang L., Willemse J.J., Ghimire L.B., van den Berg R.J.H.B.N., Janssen A.P.A., Ottenhoff T.H.M., van Boeckel C.A.A., van Wezel G.P., Ghilarov D., Martin N.I., van der Stelt M.

Nature Chemistry (2024)

Related EMDB entry:

EMD-18592

Deposited:

2024-02-01

Released:

2024-06-27

Last modified:

2024-06-27

Imageset size:

5.17 TB

Imageset DOI:

10.6019/EMPIAR-11884

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs in the EER format of LEI-800-217bp DNA - Escherichia coli gyrase complex

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

17017

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.68, 0.68)

Details:

A dose per physical pixel of 8.8 e-/s was used, resulting in a total electron dose of 39.58 e-/Å2, fractionated across 639 EPU frames. Grouping into 49 frames will result in an electron dose of 0.8 e-/Å2 per frame.

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Citations

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

CryoTRANS: predicting high-resolution maps of rare conformations from self-supervised trajectories in cryo-EM.

Fan X, Zhang Q, Zhang H, Zhu J, Ju L, Shi Z, Hu M, Bao C. (2024)

Self-assembled superstructure alleviates air-water interface effect in cryo-EM.

Zheng L, Xu J, Wang W, Gao X, Zhao C, Guo W, Sun L, Cheng H, Meng F, Chen B, Sun W, Jia X, Zhou X, Wu K, Liu Z, Ding F, Liu N, Wang HW, Peng H. (2024)

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