EMPIAR-11857 - β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 10 seconds after addition of GTP [multiple data sets in TIFF format]

EMPIAR-11857

β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 10 seconds after addition of GTP [multiple data sets in TIFF format]

Publication:

Time-resolved cryo-EM of G-protein activation by a GPCR

Papasergi-Scott MM, Pérez-Hernández G, Batebi H, Gao Y, Eskici G, Seven AB, Panova O, Hilger D, Casiraghi C, He F, Maul L, Gmeiner P, Kobilka BK, Hildebrand PW, Skiniotis G

Nature (2024)

PMID: 38480881

DOI: 10.1038/s41586-024-07153-1

Related EMDB entry:

EMD-29986 EMD-29998 EMD-42423 EMD-40020 EMD-40026 EMD-40122 EMD-40160 EMD-40175 EMD-42424 EMD-29996

Deposited:

2024-01-20

Released:

2024-06-18

Last modified:

2024-06-18

Imageset size:

12.59 TB

Imageset DOI:

10.6019/EMPIAR-11857

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 10 seconds after GTP addition (Collection 1, Grid A)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

752

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.42605, 0.42605)

Details:

Raw unaligned movie files (tif format, 77 frames, not gain corrected).
Cryo-EM imaging of the β2AR-Gs(GTP) complex was performed on a Titan Krios electron microscope equipped with a K3 Summit direct electron detector. Super-resolution pixel size: 0.42605 Å. movies were obtained at an exposure rate of 21.13 electrons/Å^2/sec. The total exposure time was 2.717 sec over 77 frames per movie stack.

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2. Unaligned multi-frame micrographs of β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 10 seconds after GTP addition (Collection 1, Grid B)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7258

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.42605, 0.42605)

Details:

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

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3. Unaligned multi-frame micrographs of β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 10 seconds after GTP addition (Collection 2, Grid B)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1949

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.42605, 0.42605)

Details:

Raw unaligned movie files (tif format, 77 frames, not gain corrected).
Cryo-EM imaging of the β2AR-Gs(GTP) complex was performed on a Titan Krios electron microscope equipped with a K3 Summit direct electron detector. Super-resolution pixel size: 0.42605 Å. Movies were obtained at an exposure rate of 20.95 electrons/ Å^2/sec. The total exposure time was 2.717 sec over 77 frames per movie stack.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Unaligned multi-frame micrographs of β2-adrenergic receptor in complex with GTP-bound G protein Gs, vitrified 10 seconds after GTP addition (Collection 3, Grid C)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

11456

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.42605, 0.42605)

Details:

Raw unaligned movie files (tif format, 79 frames, not gain corrected).
Cryo-EM imaging of the β2AR-Gs(GTP) complex was performed on a Titan Krios electron microscope equipped with a K3 Summit direct electron detector. Super-resolution pixel size: 0.42605 Å. Movies were obtained at an exposure rate of 30.71 electrons/Å^2/sec. The total exposure time was 2.008 sec over 79 frames per movie stack.

Files:

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