EMPIAR-11827 - Cryo-EM of SARS-CoV-2 5' proximal stem-loop 5 [multiple data sets in MRC format]

EMPIAR-11827

Cryo-EM of SARS-CoV-2 5' proximal stem-loop 5 [multiple data sets in MRC format]

Publication:

Tertiary folds of the SL5 RNA from the 5' proximal region of SARS-CoV-2 and related coronaviruses

Kretsch RC, Xu L, Zheludev IN, Zhou X, Huang R, Nye G, Li S, Zhang K, Chiu W, Das R

Proceedings of the National Academy of Sciences of the United States of America 121 (2024)

PMID: 38427602

DOI: 10.1073/pnas.2320493121

Related EMDB entry:

EMD-42818

Deposited:

2023-11-28

Released:

2025-07-12

Last modified:

2025-07-12

Imageset size:

34.13 TB

Imageset DOI:

10.6019/EMPIAR-11827

Experimental metadata:

Download xml json

Contains:

picked particles - single frame - processed
micrographs - multiframe

Image Sets

1. Extracted particles and orientations for final reconstruction of SARS-CoV-2 5' stem-loop 5 RNA.

Category:

picked particles - single frame - processed

Image format:

MRC

No. of images or tilt series:

445981

Image size:

(360, 360)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.82, 0.82)

Details:

Particles extracted from motion-corrected micrographs. The orientations for the final reconstruction can be found in data/SARS-CoV-2_SL5/particles/P1_J791_particles_exported.cs.

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2. Unaligned multi-frame micrographs of SARS-CoV-2 5' stem-loops 5 RNA dataset 1.

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

12633

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.82, 0.82)

Details:

These micrographs were collected with a Titan Krios G3i, 300kV and a Falcon 4 detector. Over the collection, the sample was exposed evenly to 50 electrons per angstrom squared with a total exposure time of 6.00 seconds. Desired defocuses ranged from -1.2 to -2.5 nm. The exposures are gain corrected.

Files:

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3. Unaligned multi-frame micrographs of SARS-CoV-2 5' stem-loops 5 RNA dataset 2.

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

4217

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.82, 0.82)

Details:

These micrographs were collected with a Titan Krios G3i, 300kV and a Falcon 4 detector. Over the collection, the sample was exposed evenly to 50 electrons per angstrom squared with a total exposure time of 3.84 seconds. Desired defocuses ranged from -1.2 to -2.2 nm. The exposures are gain corrected.

4.13 5.56
-1.0 to -2.0 -1.0 to -2.0
4,592 5,540

Files:

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4. Unaligned multi-frame micrographs of SARS-CoV-2 5' stem-loops 5 RNA dataset 3.

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

4592

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.82, 0.82)

Details:

These micrographs were collected with a Titan Krios G3i, 300kV and a Falcon 4 detector. Over the collection, the sample was exposed evenly to 50 electrons per angstrom squared with a total exposure time of 4.13 seconds. Desired defocuses ranged from -1.0 to -2.0 nm. The exposures are gain corrected.

4.13 5.56
-1.0 to -2.0
5,540

Files:

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5. Unaligned multi-frame micrographs of SARS-CoV-2 5' stem-loops 5 RNA dataset 4.

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

5540

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.82, 0.82)

Details:

These micrographs were collected with a Titan Krios G3i, 300kV and a Falcon 4 detector. Over the collection, the sample was exposed evenly to 50 electrons per angstrom squared with a total exposure time of 5.56 seconds. Desired defocuses ranged from -1.0 to -2.0 nm. The exposures are gain corrected.

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