EMPIAR-11814 - Cryo-EM of SARS-CoV-2 5' proximal stem-loops 5-6 with SL5c extended [multiple data sets in TIFF and MRC formats]

EMPIAR-11814

Cryo-EM of SARS-CoV-2 5' proximal stem-loops 5-6 with SL5c extended [multiple data sets in TIFF and MRC formats]

Publication:

Tertiary folds of the SL5 RNA from the 5' proximal region of SARS-CoV-2 and related coronaviruses

Kretsch RC, Xu L, Zheludev IN, Zhou X, Huang R, Nye G, Li S, Zhang K, Chiu W, Das R

Proceedings of the National Academy of Sciences of the United States of America 121 (2024)

PMID: 38427602

DOI: 10.1073/pnas.2320493121

Related EMDB entry:

EMD-42819

Deposited:

2023-11-28

Released:

2024-05-13

Last modified:

2024-05-13

Imageset size:

1.01 TB

Imageset DOI:

10.6019/EMPIAR-11814

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame micrographs of SARS-CoV-2 5' stem-loops 5-6 with SL5c extended RNA.

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5880

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.68, 0.68)

Details:

These micrographs were collected with a Titan Krios G3i, 300kV, using a BioQuantum energy filter with slit size 20eV, and a K3 detector. Over the collection, the sample was exposed evenly to 50 electrons per angstrom squared with a total exposure time of 1.19 seconds. Desired defocuses ranged from -1 to -2 nm. The exposures are not gain corrected, the gain reference can be found at data/SARS-CoV-2_SL5-6_5cext/exposures/K3-18210041 Gain Ref. x1.m1.mrc.

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2. Extracted particles and orientations for final reconstruction of SARS-CoV-2 5' stem-loops 5-6 with SL5c extended RNA.

Category:

picked particles - single frame - processed

Image format:

MRC

No. of images or tilt series:

65937

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(2.04, 2.04)

Details:

Particles extracted from motion-corrected micrographs. The orientations for the final reconstruction can be found in data/SARS-CoV-2_SL5-6_5cext/particles/P3_J1245_particles_exported.cs.

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Download a list of selected files.

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Citations

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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CryoTRANS: predicting high-resolution maps of rare conformations from self-supervised trajectories in cryo-EM.

Fan X, Zhang Q, Zhang H, Zhu J, Ju L, Shi Z, Hu M, Bao C. (2024)

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Zheng L, Xu J, Wang W, Gao X, Zhao C, Guo W, Sun L, Cheng H, Meng F, Chen B, Sun W, Jia X, Zhou X, Wu K, Liu Z, Ding F, Liu N, Wang HW, Peng H. (2024)

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