EMPIAR-11802 - AL amyloid fibril from the FOR010 light chain [4008 multi-frame micrographs composed of 963 frames each in EER format]

EMPIAR-11802

AL amyloid fibril from the FOR010 light chain [4008 multi-frame micrographs composed of 963 frames each in EER format]

Publication:

Light chain mutations contribute to defining the fibril morphology in systemic AL amyloidosis

Karimi-Farsijani S, Pfeiffer PB, Banerjee S, Baur J, Kuhn L, Kupfer N, Hegenbart U, Schönland SO, Wiese S, Haupt C, Schmidt M, Fändrich M

(---)

Related EMDB entry:

EMD-18881

Deposited:

2023-11-27

Released:

2025-02-21

Last modified:

2025-02-21

Imageset size:

1.75 TB

Imageset DOI:

10.6019/EMPIAR-11802

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of FOR010 amyloid fibrils

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

4008

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.934, 0.934)

Details:

IC-Krios1 G4 300kV C-FEG
SelectrisX 10eV slit width, Falcon4i
Cs=2.7mm, C2aperture=50um, ObjAperture=100um
EFTEM 130kx, nanoprobe spot 5, beam=630nm
Counting mode, 1pix=0.934 A
~3 s exposure, EER files save (963 fractions, not gain corrected)
~40 e/A^2 total dose

The gain reference (/data/micrographs/20220808_133943_EER_GainReference.gain) is not applied to the micrographs. The NOTES.txt file contains EM information. Thumbnails and power spectra (data/thumbnails) as well as coordinates (/data/Manual_Pick) are provided.

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Citations

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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