EMPIAR-11792 - Single-Particle Cryo-EM of DPS at Various Tilts [multiple data sets in TIFF, MRC and MRCS formats]

EMPIAR-11792

Single-Particle Cryo-EM of DPS at Various Tilts [multiple data sets in TIFF, MRC and MRCS formats]

Publication:

Overcoming Resolution Attenuation During Tilted Cryo-EM Data Collection

Aiyer S, Baldwin PR, Tan SM, Shan Z, Oh J, Mehrani A, Bowman ME, Louie G, Passos DO, Đorđević-Marquardt S, Mietzsch M, Hull JA, Hoshika S, Barad BA, Grotjahn DA, McKenna R, Agbandje-McKenna M, Benner SA, Noel JAP, Wang D, Tan YZ, Lyumkis D

bioRxiv (---)

DOI: 10.1101/2023.07.14.548955

Related EMDB entry:

EMD-36820 EMD-36818 EMD-36817 EMD-36821 EMD-36816 EMD-36822 EMD-36819

Deposited:

2023-11-27

Released:

2024-07-16

Last modified:

2024-07-16

Imageset size:

114.69 GB

Imageset DOI:

10.6019/EMPIAR-11792

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - multiframe - processed

Image Sets

1. Unaligned multiframe movies of DPS collected at various tilts

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

194

Image size:

(4092, 5760)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.834, 0.834)

Details:

Movies collected using Titan Krios and K3 quantum with energy filter. Tilt angles of 0, 10, 20, 30, 40, 50 and 60 degrees were collected put into their respective folders. 195 frames were used, pixel size is 0.834 Å/pixel, total dose of 59.6 e-/Å2. Gain reference is 20aug07b_07111315_01_4092x5760_norm_0.mrc.

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2. Aligned and dose-weighted micrographs of DPS collected at various tilts

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

194

Image size:

(4092, 5760)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.834, 0.834)

Details:

Aligned and dose weighted using UCSF MotionCor2. Tilt angles of 0, 10, 20, 30, 40, 50 and 60 degrees were collected put into their respective folders.

Files:

Archives and downloads the selected files into an uncompressed zip file.

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Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Polished and equalized particles of DPS collected at various tilts

Category:

picked particles - multiframe - processed

Image format:

MRCS

No. of images or tilt series:

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.834, 0.834)

Details:

Final particle stack after particle polishing using Relion. The particles are equalized so that there are same number of particles per tilt angle with similar defocus distribution.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

CryoTRANS: predicting high-resolution maps of rare conformations from self-supervised trajectories in cryo-EM.

Fan X, Zhang Q, Zhang H, Zhu J, Ju L, Shi Z, Hu M, Bao C. (2024)

Self-assembled superstructure alleviates air-water interface effect in cryo-EM.

Zheng L, Xu J, Wang W, Gao X, Zhao C, Guo W, Sun L, Cheng H, Meng F, Chen B, Sun W, Jia X, Zhou X, Wu K, Liu Z, Ding F, Liu N, Wang HW, Peng H. (2024)

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