EMPIAR-11714 - CryoEM movies of IAPP-S20G timecourse (3 week, FT24) [495 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-11714

CryoEM movies of IAPP-S20G timecourse (3 week, FT24) [495 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Structural evolution of fibril polymorphs during amyloid assembly

Wilkinson M, Xu Y, Thacker D, Taylor AIP, Fisher DG, Gallardo RU, Radford SE, Ranson NA

Cell 186 (2023) 5798-5811.e26

PMID: 38134875

DOI: 10.1016/j.cell.2023.11.025

Related EMDB entry:

EMD-15696

Deposited:

2023-08-03

Released:

2024-04-17

Last modified:

2024-04-17

Imageset size:

70.07 GB

Imageset DOI:

10.6019/EMPIAR-11714

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned tif movie stacks (gain corrected) for the IAPP-S20G 3 week cryoEM dataset

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

495

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.9, 0.9)

Details:

Only unaligned movies are present. The total dose was 39 e-/Å2 over 40 frames. A single polymorph was resolved (2PFp, ~30% of the fibrils).

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

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Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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