EMPIAR-11648 - Single particle cryo-EM dataset of mouse mitochondrial complex I in the active state [1235 multi-frame micrographs composed of 25 frames each in MRC format]

EMPIAR-11648

Single particle cryo-EM dataset of mouse mitochondrial complex I in the active state [1235 multi-frame micrographs composed of 25 frames each in MRC format]

Publication:

Cryo-EM structures of complex I from mouse heart mitochondria in two biochemically defined states

Agip AA, Blaza JN, Bridges HR, Viscomi C, Rawson S, Muench SP, Hirst J

Nature structural & molecular biology 25 (2018) 548-556

PMID: 29915388

DOI: 10.1038/s41594-018-0073-1

Related EMDB entry:

EMD-11377 EMD-4345

Deposited:

2023-08-02

Released:

2023-10-03

Last modified:

2023-10-03

Imageset size:

179.56 GB

Imageset DOI:

10.6019/EMPIAR-11648

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies (FoilHole*.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Compression was carried out by separating (“un-normalizing”) the gain.
The details of the method are described in
https://relion.readthedocs.io/en/release-3.1/Reference/MovieCompression.html#gain-normalised-movies.

Please apply the gain reference (gain-reference.mrc) during processing.

Original size:   1,717,300,232 kilobytes
Compressed size:   188,279,278 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Unaligned, gain-corrected micrographs of mitochondrial complex I from mouse heart

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1235

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.054, 1.054)

Details:

The micrographs are the average of 25 unaligned, gain-corrected frames.The dose rate was 5 electrons Å-2 s-1 with 10 s exposure, giving a total dose of 50 e Å-2. The data were recorded on a Krios using a K2 and energy filter (slit width 20 eV). C2 aperture of 70 μm and Objective aperture of 100 μm and defocus range of -1.5 to -3.1 μm.

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Citations

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Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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