EMPIAR-11575 - Defective HIV-1 capsid lattice assembled on 30-nm liposome scaffolds in the absence of assembly cofactor IP6 [696 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-11575

Defective HIV-1 capsid lattice assembled on 30-nm liposome scaffolds in the absence of assembly cofactor IP6 [696 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Structural insights into HIV-1 polyanion-dependent capsid lattice formation revealed by single particle cryo-EM

Highland CM, Tan A, Ricaña CL, Briggs JAG, Dick RA

Proceedings of the National Academy of Sciences of the United States of America 120 (2023)

PMID: 37094124

DOI: 10.1073/pnas.2220545120

Related EMDB entry:

EMD-29777

Deposited:

2023-04-25

Released:

2023-06-16

Last modified:

2023-06-16

Imageset size:

456.36 GB

Imageset DOI:

10.6019/EMPIAR-11575

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of HIV-1 CA lattice templated on 30-nm liposomes in the absence of assembly cofactor IP6

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

696

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.655, 0.655)

Details:

'Raw,' unaligned multi-frame movies collected using a Talos Arctica microscope equipped with a Gatan K3 detector/BioQuantum energy filter operating in super-resolution mode.

Voltage: 200 kV
Physical pixel size: 1.31Å/pixel
Total dose: 50 e-/Å^2

Gain reference (gain_ref696.mrc) is included.
Picked particle coordinates are provided in run_data.star.

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