EMPIAR-11572 - HIV-1 capsid lattice assembled on 30-nm liposome scaffolds in the presence of assembly cofactor IP6 (pH 7.4) [696 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-11572

HIV-1 capsid lattice assembled on 30-nm liposome scaffolds in the presence of assembly cofactor IP6 (pH 7.4) [696 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Structural insights into HIV-1 polyanion-dependent capsid lattice formation revealed by single particle cryo-EM

Highland CM, Tan A, Ricaña CL, Briggs JAG, Dick RA

Proceedings of the National Academy of Sciences of the United States of America 120 (2023)

PMID: 37094124

DOI: 10.1073/pnas.2220545120

Related EMDB entry:

EMD-29774

Deposited:

2023-04-25

Released:

2023-06-16

Last modified:

2023-06-16

Imageset size:

1.10 TB

Imageset DOI:

10.6019/EMPIAR-11572

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of HIV-1 CA lattice templated on 30-nm liposomes in the presence of assembly cofactor IP6 at pH 7.4

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

696

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.655, 0.655)

Details:

'Raw,' unaligned multi-frame movies collected using a Talos Arctica microscope equipped with a Gatan K3 detector/BioQuantum energy filter operating in super-resolution mode.

Voltage: 200 kV
Physical pixel size: 1.31Å/pixel
Total dose: 50 e-/Å^2

Gain reference (gain_ref.mrc) is included.
Picked particle coordinates are provided in J158_run_data.star.

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

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