EMPIAR-11546 - Cryo electron microscopy of Virus-like Particle based on PVY coat protein with dC40 deletion [6215 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-11546

Cryo electron microscopy of Virus-like Particle based on PVY coat protein with dC40 deletion [6215 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y

Kavčič L, Kežar A, Koritnik N, Žnidarič MT, Klobučar T, Vičič Ž, Merzel F, Holden E, Benesch JLP, Podobnik M

Communications chemistry 7 (2024)

PMID: 38233506

DOI: 10.1038/s42004-024-01100-x

Related EMDB entry:

EMD-17049 EMD-17050

Deposited:

2023-04-25

Released:

2024-03-26

Last modified:

2024-03-26

Imageset size:

5.82 TB

Imageset DOI:

10.6019/EMPIAR-11546

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of Virus-like Particles based on PVY coat protein with dC40 deletion

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6215

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.063, 1.063)

Details:

The movies were imaged (Titan Krios) in linear mode with Falcon2 (Thermo Fisher Scientific) direct electron detector at a nominal magnification of 75,000 corresponding to a calibrated pixel size of 1.063 Å. Each movie is collected across 40 frames with a total electron dose of 84 e-/Å2.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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