EMPIAR-11511 - Cryo-EM structure of human pseudouridine synthase 3 and tRNA-Gln [8792 micrographs in TIFF format]

EMPIAR-11511

Cryo-EM structure of human pseudouridine synthase 3 and tRNA-Gln [8792 micrographs in TIFF format]

Publication:

The molecular basis of tRNA selectivity by human pseudouridine synthase 3

Lin TY, Kleemann L, Jeżowski J, Dobosz D, Rawski M, Indyka P, Ważny G, Mehta R, Chramiec-Głąbik A, Koziej Ł, Ranff T, Fufezan C, Wawro M, Kochan J, Bereta J, Leidel SA, Glatt S

Molecular cell 84 (2024) 2472-2489.e8

PMID: 38996458

DOI: 10.1016/j.molcel.2024.06.013

Related EMDB entry:

EMD-16926

Deposited:

2023-04-03

Released:

2024-07-16

Last modified:

2024-07-16

Imageset size:

3.36 TB

Imageset DOI:

10.6019/EMPIAR-11511

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Cryo-EM structure of human PUS3-tRNA complex

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

8792

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

high tension 300kV
spherical aberration 2.7mm
pixel size 0.86 A/px
total dose on vacuum 40.06 e/A2
frames per movie 40

defocus range set -1.5, -1.2, -0.9 um

sample tilt 0 deg

imaging mode EFTEM
magnification of hole image 11500x
magnification of exposure image 105000x
beam size 0.75 um
C2 aperture 50um
objective aperture retracted

Gatan BioQuantum energy filter
energy slit 20eV
Gatan K3 counting mode
dose rate on vacuum 16.35 e/px/s
exposure image resolution 5760 x 4092
physical pixel (Super Resolution off)
image type .tiff (gain corrected)

EPU version 2.10.0.1941REL

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Citations

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

CryoTRANS: predicting high-resolution maps of rare conformations from self-supervised trajectories in cryo-EM.

Fan X, Zhang Q, Zhang H, Zhu J, Ju L, Shi Z, Hu M, Bao C. (2024)

Self-assembled superstructure alleviates air-water interface effect in cryo-EM.

Zheng L, Xu J, Wang W, Gao X, Zhao C, Guo W, Sun L, Cheng H, Meng F, Chen B, Sun W, Jia X, Zhou X, Wu K, Liu Z, Ding F, Liu N, Wang HW, Peng H. (2024)

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