EMPIAR-11339 - Cryo-EM micrographs of APOBEC3G bound to HIV-1 Vif in comblex with CBF-beta and CUL5 E3 ligase [11803 multi-frame micrographs composed of 2051 frames each in EER format]

EMPIAR-11339

Cryo-EM micrographs of APOBEC3G bound to HIV-1 Vif in comblex with CBF-beta and CUL5 E3 ligase [11803 multi-frame micrographs composed of 2051 frames each in EER format]

Publication:

Structural basis for HIV-1 antagonism of host APOBEC3G via Cullin E3 ligase

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS

Science advances 9 (2023)

PMID: 36598981

DOI: 10.1126/sciadv.ade3168

Related EMDB entry:

EMD-27875 EMD-27885 EMD-27887

Deposited:

2022-11-29

Released:

2023-01-31

Last modified:

2023-01-31

Imageset size:

11.38 TB

Imageset DOI:

10.6019/EMPIAR-11339

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of APOBEC3G and HIV-1 Vif complex with CBF-beta, ELOB, ELOC, CUL5, and RBX2

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

11803

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.92, 0.92)

Details:

We recommend dividing the total frames into 40 frame movies for motion correction. The gain reference image is in the root directory, it does not need to be flipped or rotated.

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Citations

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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