EMPIAR-11117 - Single particle cryo-EM dataset of the Vairimorpha necatrix 20S proteasome from spores [7240 multi-frame micrographs composed of 30 frames each in TIFF format]

EMPIAR-11117

Single particle cryo-EM dataset of the Vairimorpha necatrix 20S proteasome from spores [7240 multi-frame micrographs composed of 30 frames each in TIFF format]

Publication:

Structure of the reduced microsporidian proteasome bound by PI31-like peptides in dormant spores

Jespersen N, Ehrenbolger K, Winiger RR, Svedberg D, Vossbrinck CR, Barandun J

Nature communications 13 (2022)

PMID: 36379934

DOI: 10.1038/s41467-022-34691-x

Related EMDB entry:

EMD-15365

Deposited:

2022-07-15

Released:

2022-12-13

Last modified:

2022-12-13

Imageset size:

814.07 GB

Imageset DOI:

10.6019/EMPIAR-11117

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame images of the Vairimorpha necatrix 20S proteasome from spores

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7240

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.042, 1.042)

Details:

Movies with 30 frames were collected at a total dose of 35 (ds1) or 36.3 (ds2) electrons per squared angstrom second over an exposure time of 5 seconds. Defocus range of 0.7–2.8 μm, pixel size of 1.042 Å.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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