EMPIAR-11111 - Parallel cryo electron tomography (PACE-tomo) of 70S ribosomes (121 tilt series) [multiple data sets in TIFF and MRC formats]

EMPIAR-11111

Parallel cryo electron tomography (PACE-tomo) of 70S ribosomes (121 tilt series) [multiple data sets in TIFF and MRC formats]

Publication:

Parallel cryo electron tomography on in situ lamellae

Eisenstein F, Yanagisawa H, Kashihara H, Kikkawa M, Tsukita S, Danev R

Nature Methods (2022)

DOI: 10.1038/s41592-022-01690-1

Related EMDB entry:

EMD-33834

Deposited:

2022-07-12

Released:

2022-11-29

Last modified:

2022-11-29

Imageset size:

490.06 GB

Imageset DOI:

10.6019/EMPIAR-11111

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
tilt series

Image Sets

1. Raw movie frames for all tilt series

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4961

Image size:

(4092, 5760)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.07, 1.07)

Details:

camera orientation: rotation by 90, flipped around y axis

Files:

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Download a list of selected files.

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Tilt series and meta data

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

121

Image size:

(4092, 5760)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.07, 1.07)

Details:

Tilt series sorted by tilt angle, mdoc files and dose files usable in IMOD

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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