EMPIAR-11074 - Cryo-EM structure of apo-state MrgD-Gi complex [14985 multi-frame micrographs composed of 66 frames each in TIFF format]

EMPIAR-11074

Cryo-EM structure of apo-state MrgD-Gi complex [14985 multi-frame micrographs composed of 66 frames each in TIFF format]

Publication:

Structural insight into the activation mechanism of MrgD with heterotrimeric Gi-protein revealed by cryo-EM

Suzuki S, Iida M, Hiroaki Y, Tanaka K, Kawamoto A, Kato T, Oshima A

Communications Biology (---)

Related EMDB entry:

EMD-33557 EMD-33555

Deposited:

2022-06-14

Released:

2022-07-27

Last modified:

2022-07-27

Imageset size:

3.55 TB

Imageset DOI:

10.6019/EMPIAR-11074

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. K3 movies for apo-state MrgD-Gi complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

14985

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.675, 0.675)

Details:

Acceleration Voltage: 300 kV
Cs: 0.01 mm (corrected by Cs corrector)
Total Dose: 60 electron/angstrom^2
Frames: 66 frames
Energy Filter: 20 eV
Record Mode: K3 (CDS mode)
Defocus Range: -0.7 μm to -1.9 μm.

gain_220623.mrc as a gain reference without rotation and flipping.

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Citations

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

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